diff --git a/lib/mpl_toolkits/axes_grid1/inset_locator.py b/lib/mpl_toolkits/axes_grid1/inset_locator.py
index bb90f8273ce4..0771efd71fa0 100644
--- a/lib/mpl_toolkits/axes_grid1/inset_locator.py
+++ b/lib/mpl_toolkits/axes_grid1/inset_locator.py
@@ -73,7 +73,7 @@ def __call__(self, ax, renderer):
         bbox = self.get_window_extent(renderer)
         px, py = self.get_offset(bbox.width, bbox.height, 0, 0, renderer)
         bbox_canvas = Bbox.from_bounds(px, py, bbox.width, bbox.height)
-        tr = ax.figure.transFigure.inverted()
+        tr = ax.figure.transSubfigure.inverted()
         return TransformedBbox(bbox_canvas, tr)
 
 
diff --git a/lib/mpl_toolkits/axes_grid1/tests/baseline_images/test_axes_grid1/anchored_locator_base_call.png b/lib/mpl_toolkits/axes_grid1/tests/baseline_images/test_axes_grid1/anchored_locator_base_call.png
new file mode 100644
index 000000000000..31c63d7df718
Binary files /dev/null and b/lib/mpl_toolkits/axes_grid1/tests/baseline_images/test_axes_grid1/anchored_locator_base_call.png differ
diff --git a/lib/mpl_toolkits/axes_grid1/tests/test_axes_grid1.py b/lib/mpl_toolkits/axes_grid1/tests/test_axes_grid1.py
index 2dfa202cd03f..8b84f0c4e671 100644
--- a/lib/mpl_toolkits/axes_grid1/tests/test_axes_grid1.py
+++ b/lib/mpl_toolkits/axes_grid1/tests/test_axes_grid1.py
@@ -716,3 +716,23 @@ def test_removal():
     fig.canvas.draw()
     col.remove()
     fig.canvas.draw()
+
+
+@image_comparison(['anchored_locator_base_call.png'], style="mpl20")
+def test_anchored_locator_base_call():
+    fig = plt.figure(figsize=(3, 3))
+    fig1, fig2 = fig.subfigures(nrows=2, ncols=1)
+
+    ax = fig1.subplots()
+    ax.set(aspect=1, xlim=(-15, 15), ylim=(-20, 5))
+    ax.set(xticks=[], yticks=[])
+
+    Z = cbook.get_sample_data(
+        "axes_grid/bivariate_normal.npy", np_load=True
+    )
+    extent = (-3, 4, -4, 3)
+
+    axins = zoomed_inset_axes(ax, zoom=2, loc="upper left")
+    axins.set(xticks=[], yticks=[])
+
+    axins.imshow(Z, extent=extent, origin="lower")