diff --git a/crates/ruff_linter/resources/test/fixtures/pyflakes/F821_27.py b/crates/ruff_linter/resources/test/fixtures/pyflakes/F821_27.py
index 6928429d4dd5d..f9004a6dea5af 100644
--- a/crates/ruff_linter/resources/test/fixtures/pyflakes/F821_27.py
+++ b/crates/ruff_linter/resources/test/fixtures/pyflakes/F821_27.py
@@ -33,16 +33,3 @@ class MyClass:
 baz: MyClass
 eggs = baz  # Still invalid even when `__future__.annotations` are enabled
 eggs = "baz"  # always okay
-
-# Forward references:
-MaybeDStr: TypeAlias = Optional[DStr]  # Still invalid even when `__future__.annotations` are enabled
-MaybeDStr2: TypeAlias = Optional["DStr"]  # always okay
-DStr: TypeAlias = Union[D, str]  # Still invalid even when `__future__.annotations` are enabled
-DStr2: TypeAlias = Union["D", str]  # always okay
-
-class D: ...
-
-# More circular references
-class Leaf: ...
-class Tree(list[Tree | Leaf]): ...  # Still invalid even when `__future__.annotations` are enabled
-class Tree2(list["Tree | Leaf"]): ...  # always okay
diff --git a/crates/ruff_linter/src/checkers/ast/mod.rs b/crates/ruff_linter/src/checkers/ast/mod.rs
index 75b934d5ee46d..d6fb29191662b 100644
--- a/crates/ruff_linter/src/checkers/ast/mod.rs
+++ b/crates/ruff_linter/src/checkers/ast/mod.rs
@@ -937,7 +937,6 @@ impl<'a> Visitor<'a> for Checker<'a> {
             && !self.semantic.in_deferred_type_definition()
             && self.semantic.in_type_definition()
             && self.semantic.future_annotations()
-            && (self.semantic.in_typing_only_annotation() || self.source_type.is_stub())
         {
             if let Expr::StringLiteral(ast::ExprStringLiteral { value, .. }) = expr {
                 self.visit.string_type_definitions.push((
diff --git a/crates/ruff_linter/src/rules/pyflakes/snapshots/ruff_linter__rules__pyflakes__tests__F821_F821_27.py.snap b/crates/ruff_linter/src/rules/pyflakes/snapshots/ruff_linter__rules__pyflakes__tests__F821_F821_27.py.snap
index b0ef6067d4274..de22fa9f7baf9 100644
--- a/crates/ruff_linter/src/rules/pyflakes/snapshots/ruff_linter__rules__pyflakes__tests__F821_F821_27.py.snap
+++ b/crates/ruff_linter/src/rules/pyflakes/snapshots/ruff_linter__rules__pyflakes__tests__F821_F821_27.py.snap
@@ -17,30 +17,3 @@ F821_27.py:34:8: F821 Undefined name `baz`
    |        ^^^ F821
 35 | eggs = "baz"  # always okay
    |
-
-F821_27.py:38:33: F821 Undefined name `DStr`
-   |
-37 | # Forward references:
-38 | MaybeDStr: TypeAlias = Optional[DStr]  # Still invalid even when `__future__.annotations` are enabled
-   |                                 ^^^^ F821
-39 | MaybeDStr2: TypeAlias = Optional["DStr"]  # always okay
-40 | DStr: TypeAlias = Union[D, str]  # Still invalid even when `__future__.annotations` are enabled
-   |
-
-F821_27.py:40:25: F821 Undefined name `D`
-   |
-38 | MaybeDStr: TypeAlias = Optional[DStr]  # Still invalid even when `__future__.annotations` are enabled
-39 | MaybeDStr2: TypeAlias = Optional["DStr"]  # always okay
-40 | DStr: TypeAlias = Union[D, str]  # Still invalid even when `__future__.annotations` are enabled
-   |                         ^ F821
-41 | DStr2: TypeAlias = Union["D", str]  # always okay
-   |
-
-F821_27.py:47:17: F821 Undefined name `Tree`
-   |
-45 | # More circular references
-46 | class Leaf: ...
-47 | class Tree(list[Tree | Leaf]): ...  # Still invalid even when `__future__.annotations` are enabled
-   |                 ^^^^ F821
-48 | class Tree2(list["Tree | Leaf"]): ...  # always okay
-   |